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Size (kb): 2.7000000476837160

Vector: pUC-DIS (plasmid)

Promoters: Promoter lac

Construction: pUC18

Marker(s):ampR

Construct size (kb): 2.700000047683716

Features: insert detection: lacZ'
marker(s): ampR
promoter: lac
replicon: pMB1

vector for other uses

vector permitting visual detection of recombinants

Restriction digests of the clone give the following sizes (kb): SphI--2.7; SacI--2.7; KpnI--2.7; SalI--2.7.

Vector with a MCS containing only relatively rare enzyme cutting sites flanked by SfiI sites to facilitate removal of the insert. By reducing the number of sites in the plasmid, more sites in an insert can be used for gene disruption experiments.

The order of the major features in this plasmid is: SfiI/SacI/MCS/SfiI/lacZ' - pMB1 ori - ampR.

Temperature: 37.0°C