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Construct size (kb): 49.79999923706055

DESCRIPTION OF VECTOR COMPONENT:
Name of vector: Charon 4A
Intact vector size: 46.000
Type of vector: phage
Vector end: EcoRI
Vector end: EcoRI
Cloning sites: EcoRI
Polylinker sites:
Host range: Escherichia coli
Features (with orientation and position when available):
enhancer: none
promoter: none
replicon: lambda
terminator: none
Cross references:

DNA: genomic

DESCRIPTION OF INSERT COMPONENT:
Genome: Homo sapiens
Gene symbol: GC
Genomic copy number: unique
Gene name: group-specific component (vitamin D binding protein)
Contains complete coding sequence?: N
Chromosome: 4; Localization: 4 q12-q13
Tissue: fetal liver (ATCC 37333)
Type of DNA: genomic
Insert end: HaeIII or AluI Modification: EcoRI linker
Insert end: HaeIII or AluI Modification: EcoRI linker
Insert size (kb): 11.8
Cross references: DNA Seq. Acc.: L10641
Nucleotides ~4900-16900 of the insert correspond to
nucleotides ~1-12000 of L10641.

Insert lengths(kb): 11.80000019073486

Tissue: fetal liver (ATCC 37333)

Gene product: group-specific component (vitamin D binding protein)( vitamin D binding protein, vitamin D-binding alpha-globulin, group specific component) [GC]

Restriction digests of the clone give the following sizes (kb): EcoRI--22.0, 10.0, 4.5, 2.5, 1.8, 1.7, 0.7, 0.6; HindIII-->23, 18, 6.7; BglII--20, 7.2, 5.8, 4.85, 3.9, 2.8, 2.15, 1.7, 0.9, 0.65, 0.36; BamHI-->23, 3.9, 0.5; SacI-->23, 8.8, 4.7, 3.9, 1

ATCC 95662/95663 and 95664/95665 overlap by 5. kb and share EcoRI fragments of the following sizes (kb): 0.7, 1.7.

The insert contains the following restriction sites (approximate kb from the 5' end): EcoRI1 --2.5, 4.3, 4.9, 9.4, 10.1, 11.8; HindIII--1.1, 7.8; SacI--1.8, 3.3, 4.4.

Begins in the 5' flanking region and continues into intron 1.

Contains L1 (Kpn) repeats.